MYCOPATH, Vol 21, No 2 (2023)

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Evaluation of nutrient sources and in vitro efficacy of fungicides for the management of brown spot of rice

Faisal Jiskani, Muhammad Ismail, Raza Muhammad Memon, Khadim Hussain Wagan


This study reports the colonization of brown spot caused by Helminthosporium oryzae with symptomatic samples of rice and its management with fungicides. Among all the tested samples, maximum infection (84%) of H. oryzae was observed in samples from Tando Muhammad Khan while the least fungal colonization (79%) was observed in rice samples from Sijawal area. In order to get the highest recovery of fungus, different nutrient sources were also evaluated. The results showed potato dextrose agar as the best nutrient media among all other tested nutrient sources. Similarly, starch proved effective carbon source and potassium nitrate remained the best nitrogen source for fungal recovery in amended Richards agar medium. In vitro efficacy of the five fungicides showed significant reduction in fungal growth over control. The fungicide, dimethomorph + mancozeb   was very effective chemical against the fungal pathogen with 94% inhibition and difenoconazole showed the least inhibition (59%) through poison food method. The present study showed the prevalence of brown spot of rice in southern parts of Sindh. Good crop husbandry along with judicious application of fungicides can be handful to limit its further spread.

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