As the greenhouse gases are the major environmental concern worldwide and scientist consider these gases responsible for global warming. CO₂ is a major constituent of it produced by burning fossil fuel worldwide, lot of research is being done for the CO₂ mitigation purpose to preserve planet environment. Trends are shifting towards renewable energy sources and minimization of CO₂ emission to environment. Biological CO₂ fixation methods using photo-bioreactors by growing microalgae is a potential technology being explored worldwide, due to its benefits of CO₂ fixation, wastewater treatment and biofuel production it has gained world attention worldwide. As in the Middle East countries especially in Saudi Arabian region with large power plants as CO₂ emitting sources and wastewater as nutrients source for microalgae growth and higher solar irradiations to accelerate photosynthesis make it more feasible CO₂ fixation solution. In this work with the purpose of CO₂ fixation different parameters of photobioreactors affecting growth kinetics, CO₂ bio-fixation rate and biomass productivity are discussed in experimental prospective. This research will be helpful to define optimum operating parameters for culture developing of microalgae with maximum CO₂ utilization and biomass production which will contribute towards reduction of environmental concerns of CO₂.