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This study is focused on the thermodynamic performance of steam power plant based on brassica biomass as a fuel source. The power plant consists mainly of a high pressure water tube boiler of 150 ton/hr steam generation capacity, double extraction condensing steam turbine, shell and tube type surface condenser and feed water pump. The complete characterization of biomass is performed and found brassica contain higher energy per unit mass (14631 kJ/kg) compared to the other competitive available biomasses. The reason of high energy per unit mass of brassica is the presence of 38.2% and 6.7% carbon and hydrogen respectively. Furthermore, it is also reported that energy efficiency of boiler is about 80% when operated on brassica because of the higher heating value and lesser ash contents. Thermodynamic performance of power plant is further analyzed in term of exergy analysis. Based on brassica as the fuel source, the exergy efficiency is found 22% in boiler, 67% in turbine, 53% in condenser and 14% in feed water pump.