MYCOPATH, Vol 14, No 1&2 (2016)

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In vitro antagonism of Bacillus strains against Fusarium species

Nazario Francisco Francisco, *Gabriel Gallegos Morales, Yisa Maria Ochoa Fuentes, Juan Carlos Delgado Ortiz and Francisco Daniel Hernandez Castillo

Abstract


Antagonist strains of Bacillus  subtilis, B. amyloliquefaciens, B. liquefaciens and B. pumilus were evaluated for their ability to control Fusarium verticilliodes, F. oxysporum, F. acuminatum, F. solani, F. proliferatum and F. oxysporum f. sp. melonis isolated from garlic (Allium sativum L.), melon (Cucumis melo L.), and pepper plants (Capsicum annuum L.). To evaluate the antagonistic effect of different Bacillus species, mycelia plugs from the edges of actively growing fungal cultures were placed in the center of Petri dish containing potato dextrose agar. Bacillus species were inoculated at an equidistant cardinal point. After 168 h, strong growth inhibition was observed with B. pumilus (38% with F. verticilliodes). However F. oxysporum isolated from pepper plant were less inhibited (7-9%) for the four Bacillus species. Fusarium  growth inhibition by B. pumilus was evident after 120 h in dual culture.


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