The quality of tomatoes (Solanum lycopersicum L. syn name Lycopersicon esculentum) mainly depends on proper handling during post harvest processes like harvest, grading, packing and transportation. The present study was focused on shelf life of tomato based on the systematic survey of the distribution of tomato crop in markets of the district Lahore. During the survey it was evaluated that the deterioration of the produce due to packing material was 25%, transportation system was 10%, means of distribution was 5 %, exceeding post harvest losses up to 30% and sometimes the whole lot is lost. Time lag in transportation, bulky packing in the traditional wooden crates wrapped with papers cause high humidity making the microclimate favorable for mycoflora. The dominating fungi associated in decay process were Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Rhizopus stolonifer, Aspergillus fumigatus.