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Typical symptoms of Alternaria leaf necrosis were observed on leaves of gerbera daisy (Gerbera jamesonii. L) growing in a garden in Lahore, Pakistan. Pathogen was isolated from the infected leaf tissues and identified on morphological as well as molecular characteristics by nucleotide sequencing of internal transcribed spacer (ITS) region of rDNA. Isolated fungus was identified as Alternaria gaisen. The fungus was further differentiated from closely related species of genus Alternaria by phylogenetic analysis. Pathogenicity of the isolated pathogen was verified following Koch’s pathogenicity postulates. Alternaria leaf necrosis caused by A. gaisen is reported for the first time from Pakistan.