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In this study, it was aimed to determine antioxidant and antimicrobial activity of Cerrena unicolor (Bull.) Murrill which is a wood decaying mushroom. Mushroom samples were extracted with ethanol, methanol and dichloromethane in soxhlet apparatus. Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of the extracts were determined by Rel Assay Diagnostics kits. Antimicrobial activity was determined by using the modified agar dilution method against 9 bacterial and fungus strains (Staphylococcus aureus, S. aureus MRSA, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Candida albicans, C. krusei, and C. glabrata). TAS value of C. unicolor was found 6.706±0.059 mmol L^-1, TOS value was 19.308±0.114 μmol L^-1 and OSI value was 0.288±0.003. Mushroom extracts were found to be effective against microorganisms at concentrations of 25-400 µg mL^-1. In addition, the anti-candidal activity of the extracts were higher. As a result, it was determined that C. unicolor has antioxidant and antimicrobial potential.