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The aim of this study was to carry out the identification of antimicrobial compounds from the n-hexane stem extract of Kochia indica Wight by GC-MS analysis. Dried stem of K. indica was extracted in methanol and its n-hexane fraction was separated by fractionation. The GC-MS analysis of n-hexane fraction revealed the presence of 11 compounds among them hexadecanoic acid, methyl ester (24.09%) and di-n-octyl phthalate (4.49%) possess antifungal activities whereas, γ-sitosterol (13.42%) and stigmasterol (4.00%) showed antibacterial activities, while 9, 12-octadecadienoic acid, methyl ester (3.92%); β-sitosterol (18.60%) and n-hexadecanoic acid (4.57%) exhibited antimicrobial activities. In general, n-hexane stem extracts appeared to be an effective source of active antifungal and antibacterial compounds.