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Potato stem rot is wide spread disease caused fungus Neocosmospora rubicola. This disease has gained the status of emerging threat in most of the potato growing regions of the Pakistan. The investigations were conducted to evaluate the effect of nutrient media, temperature, hydrogen ion concentration and photoperiod on the growth condition of N. rubicola. The findings revealed that maximum fungal radial growth was observed at 26 ºC on PDA media having pH 7 with 16/8 light and dark period.