Ascochyta blight is a major disease of chickpea (Cicer arietinum L.) that is caused by Ascochyta rabiei (Pass.) Lab. Generally, fungicides are used to control this pathogen, which cause environmental pollution. In search of natural alternatives to these fungicides, efficacy of methanolic fruit, root-bark and stem-bark of Eucalyptus citriodora Hook. was investigated as antifungal agents against A. rabiei. Two hundred grams of dried and crushed materials of each plant part were extracted in methanol for two weeks. After evaporation of methanol on a rotary evaporator under reduced pressure, different concentrations (0.5, 1.0, 1.5, …, 4.0%) of crude methanolic extracts were prepared in malt extract broth. Fungal biomass was significantly reduced by all the concentrations of the extract. Antifungal efficacy of the extracts varied with concentrations and plant part assayed. In generally, fungal biomass production was inversely proportional to the extract concentration. Among the three plant parts, root-bark extract exhibited the highest antifungal activity followed by fruit and stem-bark extracts, respectively. There was 72–89%, 54–75% and 47–61% reduction in fungal biomass due to various concentrations of root-bark, fruit and stem-bark extracts, respectively, over negative control treatment. The present study concludes that methanolic root extract of E. citriodora possesses substantial antifungal potential to control in vitro growth of A. rabiei.