Canola is a cash commercial crop renowned for its edible oil production ability. Brown and black spot diseases of stem and leaves of canola plants caused by Alternaria species have always been a threat for their growth and productivity ultimately economic loss. Several strategies have been adopted to control these fungal pathogens, while, use of commercially available chemical fungicides is the most common. For this in present study, two chemical fungicides with trade name Triton and Benedict contains active ingredients validamycin and iprobenfos, respectively were evaluated for their ability to control the canola spot disease pathogen, Alternaria sp. Fungus was grown on growth media incorporated with fungicides by three different methods viz. well diffusion, disc diffusion and food poisoning. Maximum inhibition in fungus growth was recorded by food poisoning method using either of fungicide. Whereas, Benedict (iprobenfos) was more effective against Alternaria sp. as compared to Triton (validamycin). Therefore, this method is suggested for researchers being more efficient for laboratory assays.