In vitro toxic influence of Cr(VI) was studied on seed germination and seedling growth of three pulse species namely mungbean [Vigna radiata (L.) Wilczek], mashbean [Vigna mungo (L.) Hepper], and cowpea [Vigna unguiculata (L.) Walp ]. Cr(VI) toxicity effect was also assessed on Macrophhomina phaseolina (Tassi) Goid, the cause charcoal rot disease in pulses and other field crops. Six different concentrations viz. 50, 100, 200, 300, 400 and 500 ppm of Cr(VI) were applied on seeds of  the pulses in 9-cm diameter Petri plates lined with filter papers. All the six concentrations of Cr(VI) caused a significant reduction of 5–20%, 4–20% and 5–22% in germination, and root and shoot length, respectively. Higher concentrations (300–500 ppm) of metal solution caused more inhibition in investigated parameters as compared to lower concentrations (50-200 ppm). The sensitivity of the three pulses towards metal ions was found in order of: V mungo > V. unguiculata > V. radiata. Toxicity of Cr(VI) against M. Phaseolina was evaluated on 2% malt extract agar having nine Cr(VI) concentrations viz. 25, 50, 75, 100, 150, 200, 300, 400, and 500 ppm. Growth of the pathogenic fungus was markedly reduced by 20-90% at lower concentrations of 25 to 150 ppm and the fungus was unable to grow with further increasing metal concentrations. The present study concludes that early growth of three pulse species as well as their pathogen is very sensitive to Cr(VI). The contemporary research recommends for the elaborated investigation on involvement of plant pathogens and heavy metal on the growth and development of the economically important edible crops growing near industrial areas.