This manuscript reports essential oil extraction from the flower petals of Rosa gruss-an-teplitz by distillation and solvent extraction methods, and its physico-chemical analysis. Yield of concrete oil was 0.202 and absolute oil 0.199 %, on the basis of fresh flower weight, respectively, whereas absolute oil was 9.827% on concrete oil basis. The extracted oil was yellowish in colour with refractive index 1.43 at 25 °C, congealing point 15 °C, optical rotation -32.50 + 45.25, specific gravity 0.9 at 20 °C, acid number 12 and ester number 25.22. The chemical components of the essential oil of R. gruss-an-teplitz were determined by Gas Liquid Chromatography (GLC). Fifteen compounds were identified in essential oil. Major constituents were phenyl ethyl alcohol (49.31%), geranyle acetate (14.78%), citronellol (12.09%), benzyl acetate (0.27%) and citronellyle acetate (12.18%).