Tomato leaf curl Palampur virus (ToLCPMV) is a bipartite begomovirus, which causes losses to several crops. In this research, we evaluated the effect of biofertilizer Phosphozist containing Serratia marcescens, on tomato resistance to ToLCPMV. Expression of PR5 and PR13 genes were evaluated using real-time PCR technique. Phosphozist-treated plants had significantly lower disease severity than the control. The control rate of ToLCPMV was 32.67%. The level of expression of PR5 and PR13 genes was significantly increased by biofertilizer Phosphozist. The results of this research indicated that S. marcescens reduced the severity of ToLCPMV disease and increased the expression of PR5 and PR13 genes in tomato plants and this reduction of the disease severity by S. marcescens was probably due to induction of resistance genes.