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Entomopathogenic fungi are emerging as an effective management method as compared to potential snags created by chemical insecticides that includes environmental pollution and the development of resistance. Many entomopathogenic fungi are in use as a bio-control agent while different insect associated fungi are isolated from insects. The goal of this study was to check the infection mechanism of Fusarium and Aspergillus species on 4th instar of Bemisia tabaci isolated from insects previously. Both genera showed the efficient results against 4th instar of B. tabaci with growth retardation and severe infection in insect body. Directly spores of Fusarium and Aspergillus species were applied on 4th instar of B. tabaci and data regarding duration of spore germination, penetration, colonization and infection was determined under control conditions. It was observed that spores of Aspergillus species germinate quickly as compare to Fusarium species. There was no significant difference in spore germination on species level. Penetration time of Aspergillus and Fusarium species vary and colonization and infection duration of Aspergillus species was less as compared to Fusarium species used in this study. In this study, it was observed that Aspergillus is more aggressive as compare to Fusarium and also it depends on the isolates of insects.