A feeding study was conducted for 120 days on chicks (Gallus gallus L.) to evaluate effect of two varieties of transgenic cotton containing Bt (CEMB-Cry1Ac +Cry2A) and GTG gene (EPSPS enzyme) on growth, egg productivity and clinical chemistry of blood, serum protein and histology of organs. A total of 90- and 15-days old layer chicks were taken and divided in three groups each with three replicates. Two types of diets were given to chicks i.e. transgenic cotton with Cry1Ac+Cry2A gene, cotton leaves containing GTG gene and non-transgenic cotton served as control. Birds weighed at fifteen days intervals showed no significance difference in their weight. Egg production rate was significantly different in the different treatments. Clinical chemistry of blood, serum protein and histological analysis of organs (heart, liver, spleen and intestine) showed no significant difference in the transgenic diet and control diet fed animals.