Penicillium expansum is a common soil-borne fungus occurring worldwide and causes post harvest diseases in a wide range of plant species especially in apple. Three fungicides namely metalaxyl+mencozeb 72% WP, thiophenate methyl 70% WP and fosetyl-Al 80% WP were evaluated in vitro against this fungal pathogen. Five concentrations of each fungicide viz. 50, 100, 150, 200 and 250 ppm, were tested by food poisoning technique using malt extract agar as a growth medium. All the three fungicides significantly reduced the pathogen growth with variable extents. Among these, thiophenate methyl was the most effective one causing 65–90% reduction in fungal growth. Metalaxyl+mencozeb also showed a very similar effect resulting in 73–88% suppression in the fungal growth. Fosetyl-Al was comparatively less effective fungicide and reduced fungal growth by 73–88%, over control. This study concludes that thiophenate methyl and metalaxyl+mencozeb are highly effective fungicides against P. expansum, whose 50 ppm concentration can control fungal growth by 65 and 73%, respectively.