The aim of this study was to analyze essential oil from flowers of Paulownia fortunei through GC-MS for identification of possible antimicrobial constituents. The essential oil from flowers of P. fortunei was extracted by microwave assisted technique. The oil was analyzed by GC-MS that showed the presence of six compounds. Nerolidol was the principal compound in the extract with 82.81% peak area. Other compounds included pentacosane (3.95%), octadecane (4.77%), 5,9-undecadien-2-one, 6,10-dimethyl-, (E)- (3.02%), heptacosane (2.81%), and (E,E,E)-3,7,11,15-tetramethylhexadeca-1,3,6,10,14-pentaene (2.68%). Literature survey showed that the principal compound nerolidol possesses potent antibacterial and antifungal properties.