The oyster mushroom, Pleurotus florida (Strain PK-401) was cultivated on different cereal straws for early and high yielding crop. The wheat and paddy straw, empty corn cobs and millet heads were used as substrate. The earlier pinhead formation and maturation of fruiting bodies were observed in case of empty corncobs, followed by paddy straw, wheat straw and empty millet heads, respectively. The maximum number of flushes was harvested from wheat and paddy straw followed by empty corncobs and millet heads. The less period between flushes was recorded on wheat and paddy straw followed by empty corncobs and millet heads. The maximum fresh yield on percentage of substrate dry weight basis was obtained from wheat and paddy straw followed by empty corncobs and millet heads.