The effect of culture media, carbon and nitrogen sources, pH levels and temperature were studied on mycelial growth of Ascochyta rabiei. Maximum growth of the fungus was found on chickpea extract agar medium. Glucose was found to be the best source of carbon while potassium nitrate and sodium nitrate were better sources of nitrogen. The most suitable pH level for growth of the fungus was 7.0 and 7.5. Growth of A. rabiei was maximum at 25⁰ C after 15 days of inoculation while it is not significantly different from that of 20⁰C after 21 days of inoculation. The growth was reduced drastically below 10⁰C and above 30⁰C.