The incidence and frequency of fungi on safflower seed with their role in seed germination was investigated. Three samples each of five safflower cultivars/lines were collected from National Oil Development Programme (NODP), NARC Islamabad. Eleven different fungal species belonging to seven genera i.e., Alternaria, Aspergillus, Chaetomium, Curvularia, Fusarium, Helminthosporium and Rhizopus were observed. The incidence and frequency of these fungi varied with cultivar and lines with maximum fungal prevalence in Thori-78 (7.7) and minimum in Dholka Sindh (5.4). Maximum germination was noticed on Dholka Sindh (85%) with least fungal incidence (23%) and minimum seed germination was observed on Thori-78 (60%) with higher fungal incidence (55%).