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Different culturing medium viz. malt extract agar, potato dextrose agar, waksman agar, saboured agar and corn meal agar were investigated for the effective mycelial running of Shiitake mushroom [Lentinula edode (Berk) Pegler]. Maximum mycelial growth after 3, 6 and 9 days was observed on potato dextrose agar. Minimum mycelial growth after 3, 6 and 9 days was observed on waksman agar. Data regarding pH 5, 6, 7, 8 and 9 of growth medium was also recorded after 3, 6 and 9 days. Maximum mycelial growth was observed at neutral pH to slightly acidic (6). Maximum growth of 8 cm after nine days was recorded on potato dextrose agar medium followed by malt extract (7.58 cm), corn meal agar (5.35 cm), saboured agar (4.2 cm) and waksman agar (3.75 cm). This study concludes that potato dextrose agar and 6 pH are the best for the effective mycelial growth of Shiitake mushroom.