In this study, 309 myxomycete species reported so far in Türkiye are listed. Previously, in the checklist published in 2019, 286 species were reported. With the addition of new records and the removal of synonyms, the number of species have reached to 309 today. These species belong to 5 orders, 12 families and 45 genera. Physarida and Physarum are the largest order and genus, respectively, containing the highest number of species. Türkiye is not poor in terms of myxobiota. However, studies on Myxomycetes in this country are still insufficient.