Infection of the skin is caused by various microbial agents such as fungi. Given the alarming spread of microbial resistance, a new therapeutic approach to combat antibiotic-resistant microbes such as nanoparticles seems necessary. The objective of this study was the investigation of properties of silver nanoparticles synthesized by a mixed extract of Scrophularia striata and Urtica dioica and to evaluate their biological activity against skin infectious fungi. ultra violet (UV) spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Energy-Dispersive X-ray (EDAX) and scanning electron microscopy (SEM) were used for characterizing the synthesized nanoparticles. Antimicrobial activity of the nanoparticles was then evaluated against three dermatophytes namely Microsporum canis, Trichophyton rubrum and Candida albicans by measuring the growth disc diameter method. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were also determined using microdilution technique. The maximum absorption peak of the nanoparticles was observed at 450 nm, which is in the range of absorption for silver nanoparticles. X-ray diffraction also confirmed the presence of nano silver crystals. The electron microscopy imaging showed that the nanoparticles were spherical in shape and have an average size of 40±5 nm. MIC value for biosynthesized nanoparticles were 31, 15 and 7 ppm for T. rubrum, M. canis and C. albicans, respectively. It is suggested that the silver nanoparticles synthesized by using a combination of U. dioica and S. striata extracts could be used as potent antifungal agents against the skin-deep pathogenic fungi.