Ganoderma lucidum, (Curtis) P. Karst is a white rot fungus belongs to basidiomycetes group, distributed throughout world. In present study, G. lucidum was used to assess laccase production under varying culture conditions like inoculum size, temperature, pH and incubation period. Maximum laccase enzyme activity was obtained at 6 pH, 25 °C at 7^th day of incubation with 5 disc of inoculums.