In this study, the antifungal activity of Anoectangium clarum Mitt. and Hyophila spathulata (Harv.) A. Jaeger. was tested against three different fungal species viz. Aspergillus niger van Tieghem, Fusarium solani (Mart.) Sacc. and Trichoderma viride Pers. Five extraction solvents were used, i.e. acetone, chlororform, distilled water, ethanol and methanol. The activity was tested by the disc-diffusion method. Ethanol and methanol extracts at concentrations of 4% and 5% of the selected mosses showed significant antifungal activity against the fungal strains. In more specific and comparative manner, it is concluded here that Hyophila spathulata was even more effective antifungal agent than Anoectangium clarum.