In the present study, in vitro antagonistic potential of seven species of genus Trichoderma namely T. viride Pers., T. harzianum Rifai, T. koningii Oudem, T. pseudokoningii Oudem, T. aureoviride Pers., T. hamatum Bain and T. reesei Rifai was assessed against a destructive soil-borne plant pathogen Sclerotium rolfsii Sacc., the cause of collar rot disease of chickpea (Cicer arietinum L.). Trichoderma species were screened by dual culture method in which cultures of the pathogenic and antagonistic fungi were grown side by side on the growth medium in the same Petri plate. T. viride, T. harzianum, T. koningii and T. pseudokoningii exhibited pronounced antagonistic activity against the pathogenic fungal species resulting up to 40-68% reduction in its growth. T. viride showed the best antagonistic behavior approximately followed by T. harzianum causing 68% and 57% reduction in pathogen growth. Besides, the percentage reduction in number of sclerotia of S. rolfsii due to interactions with different Trichoderma species was also recorded. The present study concludes that T. viride has the best antagonistic potential against S. rolfsii followed by T. harzianum.