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The current research aimed to explore the secondary metabolites and antibacterial activity of extracts prepared from peel part of Punica granatum L., family Punicaceae. The extract was prepared in different solvents including water, methanol, ethanol, petroleum ether and ethyl acetate. The antibacterial activity was examined against one gram positive (Staphylococcus aureus) and two gram negative (Escherichia coli and Pseudomonas aeruginosa) ATCC bacterial strains on the basis of zone of inhibition using agar well diffusion assay. Results revealed that maximum number of the phytochemical molecules were existing in methanolic, ethanolic and ethyl acetate, while minimum number of molecules were found in aqueous solvent. P. granatum exhibits significantly high activity against both gram negative and gram positive bacteria.