MYCOPATH, Vol 13, No 2 (2015)

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Growth assessment of three common soil fungi (Trichoderma viride, Aspergillus niger and Penicillium sp.) on formulated culture medium

* M. Ubogu, E. Akponah and F.E. Ejukonemu

Abstract


The radial mycelial extension growth, mycelia extension growth rate and minimum sporulation time of three common soil fungi namely Trichoderma viride, Aspergillus niger and Penicillium sp. were investigated on  Irish potato (Solanum tuberosum L.), cocoyam [Xanthosomas sagittifolium (L.) Schott], sweet potato [Ipomoea batatas (L.) Lam.], cassava (Manihot esculenta Crantz), yam (Dioscorea rotundata Poir.), shorgum [Sorghum bicolor (L.) Moench], rice (Oryza sativa L.), corn (Zea mays L.), millet (Panicum miliaceum L.), wheat (Triticum aestivum L.) based formulated culture medium in comparison to commercially produced packaged dehydrated potato dextrose agar (PDA) for a period of 96 h. All the formulated mediumsupported growth of the three fungi within the period of investigation. Sweet potato, Irish potato and corn based formulated culture medium consistently produced mycelial extension growth of T. viride, A. niger and Penicillium sp. that was remarkably higher than that of commercially produced PDA. The mycelial extension growth of T. viride and Penicillium sp. on tuber-based formulated medium were statistically the same with that of grain-based medium, while A. niger showed higher growth on grain-based medium. The minimum sporulation time for all the test fungi was 48 h in commercially produced PDA. While the minimum sporulation time for A. niger was 48 h, and that of T. viride and Penicilium sp. ranged from 48-96 h on the formulated medium.


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