This study was conducted to evaluate the role of native trees of Lahore in absorbing heavy metals and to evaluate antifungal potential of leaf extracts of these tree species. Azadirachta indica, Dalbergia sissoo, Eucalyptus citriodora, Nerium oleander and Polyalthia longifolia were assessed for their ability to accumulate chromium (Cr) and lead (Pb) through foliar routes. A comparative analysis was conducted between leaf samples collected from botanical gardens (control) and heavy traffic zones (experimental), focusing on detailed leaf tissue morphology, chlorophyll content and heavy metal concentrations. E. citriodora and N. oleander exhibited the highest Cr and Pb accumulation potential while P. longifolia demonstrated a steady lower level of metal accumulation ability.  Antifungal assays revealed the highest activity of E. citriodora and A. indica extracts against Macrophomina phaseolina followed by D. sissoo, P. longifolia, and N. oleander. These findings advocate for strategic planting of hyperaccumulator and antifungal tree species to mitigate pollution and enhance urban environmental health.