The focus of this work is to study the effect of the cotton fibers on the starch/ Poly (vinyl alcohol) (PVA) blends films. The biodegradable composites films were prepared by Starch, PVA and Natural cotton fibers. Starch and PVA blend films were prepared using solution casting method. The starch/PVA/cotton fibers films samples were analyzed for the tensile, chemical and morphological properties. Experimental results showed that the incorporation of the short cotton fibers into the starch/PVA matrix caused a reasonable improvement in of the tensile modulus (18.6 times) and strength (2.07 to 4.38MPa). There was also improvement in the elongation (104.56 to 8.82%) and impact (28.4%) properties. Chemical and structure analysis were done using Fourier Transform Infrared Spectroscopy (FTIR) and surface morphology were studies by optical microscope.