Effect of nitrozist bio-fertilizer on melon resistant to wilt disease caused by Fusarium oxysporum f.sp. melonis and expression of melon defense-related genes
Abstract
Fusarium oxysporum f. sp. melonis (Fom) is causal agent of the Fusarium wilt of melon, with a reported loss of 100% on melon (Cucumis melo L.). This research was conducted to study the effect of nitrozist bio-fertilizer (containing Enterobacter sp.) on melon resistant to wilt disease caused by Fom. This experiment was carried out as seed treatment with nitrozist bio-fertilizer under greenhouse conditions. Furthermore, the expression of peroxidase (POX), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL) and lipoxygenase 2 (LOX2) genes was evaluated using Real-Time PCR (qRT-PCR) technique. Treated plants with nitrozist bio-fertilizer had a significant difference with regard to the severity of the disease. The percentage of disease reduction of nitrozist bio-fertilizer was 41%. The level of expression of POX, PPO, PAL and LOX2 genes was significantly increased by nitrozist bio-fertilizer. Therefore, the use of a nitrozist bio-fertilizer containing Enterobacter sp. is efficient in induction of resistance in melon plants against to Fom.
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