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In this study, antioxidant potantial, oxidant potantial, oxidative stress index and heavy metal contents of Daedaleopsis nitida (Durieu & Mont.) Zmitr. & Malysheva mushroom were determined. Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were determined using Rel Assay Diagnostics kits. Cr, Cu, Mn, Fe, Ni, Cd, Pb and Zn contents of fruiting bodies of mushroom were determined by atomic absorption spectrophotometer. As a result of the study, TAS value of mushroom extract was determined as 6.072 ± 0.067, TOS value was 7.165 ± 0.074 and OSI value was 0.118 ± 0.002. Cr, Cu, Mn, Fe, Ni, Cd, Pb and Zn contents of the fruiting bodies of mushroom were determined 16.87 ± 0.73, 109.95 ± 2.17, 8.14 ± 0.81, 239.54 ± 14.30, 1.91 ± 0.64, 7.05 ± 0.83, 0.68 ± 0.31 and 55.96 ± 1.07, respectively.