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Cultivation of mushrooms on lignocellulosic wastes represents one of the most economic organic recycling processes. In this research, three Pleurotus species viz., P. nebrodensis, P. ostreatus and P. eryngii were cultivated on cotton waste, saw dust, paddy straw and mixture of these substrates (cotton waste + saw dust, cotton waste + paddy straw, saw dust + paddy straw + cotton waste). Mycelial growth (days), stalk length and diameter (mm), emergence of primordia (days), time period from primordial initiation to harvesting (days), number of fruiting bodies, average individual weight of fruiting bodies (g), number of flushes, total yield (kg), interval between flushes (days) and biological efficiency were recorded. Amongst the three fungi, P. ostreatus showed the best growth and productivity. Saw dust gave the best results in spawn running, time interval between primordial initiation to harvesting stage and in number of flushes. Combination of saw dust, paddy straw and cotton waste gave the best results in emergence of primordia, fruiting bodies weight, moisture percentage, biological efficiency and total yield.