The aim of this study was to evaluate the impact of arbuscular mycorrhizal (AM) fungus Glomus mosseae Gerd. and Trappe on plant growth and development, including root proliferation, chlorophyll, and carotenoid content in three black gram [Vigna mungo (L.) Hepper] accession NULL-7, MLT-9 and MLT-10. The study revealed that the G. mosseae inoculated mycorrhizal plants had the comparatively higher percentage of mean plant height 9% to 31.25% root length 15.64% to 28.83% plant fresh biomass 18.20% to 29.70%, root biomass 18.44 to 25.53, total chlorophyll 8.23% to 10.43%, and carotenoid levels 24.05% to 33.33%, chlorophyll A 7.74% to 22.88 % and Chlorophyll B 8.16% to 19.01% as compared to non-mycorrhizal control plants. The present findings suggest that symbiotic association between G. mosseae, and V. mungo have substantially affected various plant growth parameters and photosynthetic pigments, which might be important and promising for long-term black gram production.