A collection of 16 basidiomycetes was carried out in northwestern Algeria. During the harvest, the average temperature of the sites was between 11.2 to 19.3 °C with an average humidity of 67 to 90%.  The dominant plants observed at the sampling sites are pine, oak and false pepper. The collected mushrooms were identified by their macroscopic, microscopic (light microscope and scanning electron microscope) and macrochemical characteristics using chemical reagents to distinguish among the different species. Of the 16 fungi, a genotypic identification was made using the tools of molecular biology and bioinformatics analysis.  The results of the phenotypic analysis showed that the fungi in question were: Lactarius zonarioides, Amanita proxima, A. virosa, Agaricus bisporus, Suillus mediterraneensis, Boletus sp., Xerocomus sp., Coprinus atramentarius, Pleurotus ostreatus, P. pulmonarius, P. eryngii, P. dryinus, Lepiota sp., Cortinarius orellanus, Armillaria mellea and Trametes quercina. The comparison of the ITS1 sequences (143 bp) with those of the databases was carried out using the BLAST algorithm, which confirmed the identification of Agaricus bisporus.