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In vitro physiological studies on mycelial growth of Macrophomina phaseolina (Tassi) Goid revealed that the fungus grew best on cornmeal agar out of five culture media that were tried. All the carbon sources were found to be more or less equally good while peptone was the best among the nitrogen sources. Growth of M. phaseolina was maximum at 300 C after 7 days of inoculation, which was reduced significantly below 200 C and above 350 C. All the tested pH levels (6.0 to 8.0) were found equally suitable for growth of fungus.