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The present investigation deals with antimicrobial activity of ethanolic extracts of Cuscuta reflexa parasitizing on three different angiospermic hosts.namly Populus euro- Americana ,Zizphus hysudrica and Clerodendron inerme .The extracts were reported to exhibit significant antimicrobial activity against bacteria like Pseudomonas aeruginosa , Escherichia coli, Bacillus subtilis and Bacillus licheniformis, and fungi namely Aspergillus niger and Trichoderma reesei. The results when compared with the inhibition caused by standard reference discs of antibiotics for example Amikacine, Ciprofloxacin and Griseofulvin showed significant differences among three hosts with respect to antimicrobial activity.