Chili (Capsicum annuum L.) is among the major vegetables of Punjab. Bacterial wilt caused by Ralstonia solanacearum (Smith) Yabuuchi is responsible for significant reduction in its production. In the present study, incidence and prevalence of this disease on chili were determined and biovars were identified in  five major chili growing districts of Punjab, Pakistan namely Multan, Kasur, Pakpattan, Bahawalpur and Attock. The study revealed an overall 11% incidence and 85% prevalence of bacterial wilt in Punjab. The highest disease incidence of 16% was found in Pakpattan district followed by Kasur (12%) and Attock (12%). Disease incidence was found to be minimum in Multan and Bahawalpur districts (9% each). Prevalence of bacterial wilt was 90% in Pakpattan and Kasur and 80% in rest of the districts. Out of 42 R. solanacearum strains isolated from various localities, 82% were identified as Biovar 3, while the remaining 18% were recognized as Biovar 4. Biovar 3 was recorded from all the districts while Biovar 4 was present in all districts except Attock. As bacterial wilt is widely prevalent throughout the province with varying intensities; therefore, stringent surveillance and control measures are needed to preclude the spread and severity of the disease.