The aim of the study was to examine the impact of several strains of Trichoderma on soybean [Glycine max (L.) Merr.] growth promotion. The effects of three Trichoderma spp. viz. T. hamatum, T. harzianum and T. viride were examined both singly and in combination on growth and physiology of soybean. The growth-promoting effects of the Trichoderma spp. was estimated through morphological features, antioxidant assay, chlorophyll content, and nutritional contents (N, P, and K) estimation. The best morphological characteristics were displayed by the T. hamatum-inoculated plant, either singly or in combination. Combined application of T. harzianum + T. hamatum resulted in maximum total chlorophyll content. T. hamatum inoculation also resulted in maximum antioxidants potential through estimation of total phenolic content. Likewise, the highest antioxidant potential was found in the T. hamatum + T. harzianum inoculation as shown by total antioxidant and DPPH assays. It concluded that inoculation with selected plant growth promoting fungi could serve as a useful tool for soybean crop in Pakistan.