Boletes in the broadest sense belong to many genera and even families. They are mushrooms with soft, putrescible flesh, sometimes turning blue on contact with air. They generally grow on the ground, close to trees, where they develop mycorrhizae. In Burkina Faso, these mushrooms contribute significantly to the food supply of some populations, as well as to the regeneration of forest ecosystems through their ectomycorrhizal relationships. The present study was carried out in the gallery forests of Dan, Toussianbandougou and Kotoudeni in Burkina Faso. The objective of this study was to identify different species of boletes in this area by describing their microscopic and macroscopic characteristics. Macroscopic, microscopic and anatomic studies led to identification of three species of boletes namely Boletellus lindéri, Tylopilus balloui and Strobilomyces strobilaceus. Analysis of spore measurements showed a significant difference among the three species. Of these species, Strobilomyces strobilaceus was identified as endemic to Burkina Faso.