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The present study aimed to develop a high performance thin layer chromatography method for the determination of sinensetin and apply it to standardize the extracts Orthosiphon stamineus. The samples and a series of standards were applied in duplicate on silica gel plate, which was then developed using mobile phase comprising chloroform and ethyl acetate (6:4, v/v) to a distance of 5 cm. Afterwards, the plate was dried and subjected to densitometry at 366 nm for the quantification of sinensetin. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 0.05 and 0.50 µg/ml, respectively. The method showed linearity in a range of 0.50-100.00 µg/ml with correlation coefficient 0.9988. The recovery, intra- and inter day accuracy were found to be 95.67-97.65, 97.64-99.54 and 97.83-98.57%, respectively, with relative standard deviation (RSD) less than 5%. The extracts contained sinensetin from 0.470-1.335 mg/g/. The results of this study indicate that the developed method is sensitive, reliable, repeatable and reproducible and may be applied to standardize extracts of Orthosiphon stamineus.